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mastercycler ep gradient s  (Eppendorf AG)


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    Structured Review

    Eppendorf AG mastercycler ep gradient s
    Mastercycler Ep Gradient S, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 200187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mastercycler+ep+gradient+s/Eppendorf/pm42070534-137-13-17
    Average 99 stars, based on 200187 article reviews
    mastercycler ep gradient s - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Phylogenetic position and new locality record of Salsuginus seculus (Dactylogyridae) associated with Gambusia affinis in its native range in the USA
    Article Snippet: PCR reactions consisted of 1 U of Taq DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA), 1 × PCR buffer, 1.5 mM MgCl2, 0.4 mM of each dNTP, 0.4 μM of each primer, and an aliquot of 1 μL of genomic DNA in a total volume of 25 μL. .. PCR was carried out in a Mastercycler ep gradient S (Eppendorf AG, Hamburg, Germany) with the following steps: 2 min at 94 °C followed by 39 cycles of 45 s at 92 °C, 90 s at 48 °C, and 105 s at 72 °C, and 7 min of final elongation at 72 °C. .. The PCR product was purified using EPPiC Fast (A & A Biotechnology, Gdynia, Poland) and then sequenced in both directions using the same primers as those used in the amplification reaction.

    Article Title: Somatic embryogenesis in Vaccinium vitis-idaea L.: a high-fidelity micropropagation approach producing true-to-type plants with enhanced phytochemical profiles
    Article Snippet: .. The homogenates were prepared using in total, was composed of 2 μL of 25 ng of DNA template, 10 μL of Phusion green hot start II PCR master mix (high fidelity PCR master-mic) sourced from Thermo Scientific), 2 μL of both the forward and reverse primers, along with 7 μL of PCR-grade distilled water from Sigma Chemical Co. DNA amplification was conducted using a Mastercycler ep Gradient S (Eppendorf AG, 22,331 Hamburg, Germany) with a program consisting of an initial denaturation step at 94 °C for 10 min to facilitate a'hot start'. ..

    Article Title: Tracing the Uncharted African Diaspora in Southern Brazil: The Genetic Legacies of Resistance in Two Quilombos from Paraná
    Article Snippet: .. The PCR products were purified with the enzymes exonuclease I (Fermentas, Vilnius, Lithuania) and alkaline phosphatase (Thermo Fisher Scientific, Waltham, MA, USA) on a Mastercycler EP Gradient S (Eppendorf) at 37 °C for 1 h and 80 °C for 15 min. .. The purified products were then amplified using BigDye Terminator Cycle Sequencing Standard v3.1 (Life Technologies, Carlsbad, CA, USA), according to the manufacturer’s instructions, and using the same primers.

    Reverse Transcription:

    Article Title: Adipokines in Breast Cancer: Decoding Genetic and Proteomic Mechanisms Underlying Migration, Invasion, and Proliferation
    Article Snippet: Using the PureLink RNA Mini Kit (Invitrogen, #12183025) and PureLink DNase-Set (Invitrogen, #12185-010) the RNA was isolated, and its quantity determined with the Qubit RNA HS Assay Kit (Invitrogen, #Q32852). .. With the help of Reverse Transcriptase (Applied Biosystems, #4308228), 1 μg of RNA was transcribed into cDNA in the Mastercycler EP Gradient S (Eppendorf, #S5345) and its amount was determined leveraging the Qubit ssDNA HS Kit (Invitrogen, Q10212). ..

    DNA Amplification:

    Article Title: Somatic embryogenesis in Vaccinium vitis-idaea L.: a high-fidelity micropropagation approach producing true-to-type plants with enhanced phytochemical profiles
    Article Snippet: .. The homogenates were prepared using in total, was composed of 2 μL of 25 ng of DNA template, 10 μL of Phusion green hot start II PCR master mix (high fidelity PCR master-mic) sourced from Thermo Scientific), 2 μL of both the forward and reverse primers, along with 7 μL of PCR-grade distilled water from Sigma Chemical Co. DNA amplification was conducted using a Mastercycler ep Gradient S (Eppendorf AG, 22,331 Hamburg, Germany) with a program consisting of an initial denaturation step at 94 °C for 10 min to facilitate a'hot start'. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Unraveling effect pathways and biomagnification: The impact of a polyfluoroalkyl fungicide on an aquatic decomposer-detritivore system.
    Article Snippet: DNA was extracted from leaf material of known dry weight (nearest 0.01 mg) using the FastDNA® Spin Kit for Soil (MP Biomedicals, Schwerte, Germany) following the manufacturer's instructions. .. Afterward, qPCR reactions were performed to estimate fungal and bacterial abundance using a Mastercycler ep gradient S (Eppendorf, Germany), following the procedure described in Feckler et al. (2023). ..

    Article Title: Effects of cyanobacterium Phormidium nigroviride K3 on zebrafish embryos and genomic insights into its toxic potential.
    Article Snippet: Cyanobacterial blooms in freshwater systems pose significant environmental and public health risks, largely due to their production of toxic secondary metabolites.. This study investigated the effects of Phormidium nigroviride K3 extracts on early zebrafish development, focusing on organismal toxicity and gene expression changes.. Acute toxicity was assessed by monitoring developmental deformities and evaluating relative changes in the expression of six genes associated with xenobiotic metabolism, apoptosis, endoplasmic reticulum stress, and endocrine processes.

    Purification:

    Article Title: Tracing the Uncharted African Diaspora in Southern Brazil: The Genetic Legacies of Resistance in Two Quilombos from Paraná
    Article Snippet: .. The PCR products were purified with the enzymes exonuclease I (Fermentas, Vilnius, Lithuania) and alkaline phosphatase (Thermo Fisher Scientific, Waltham, MA, USA) on a Mastercycler EP Gradient S (Eppendorf) at 37 °C for 1 h and 80 °C for 15 min. .. The purified products were then amplified using BigDye Terminator Cycle Sequencing Standard v3.1 (Life Technologies, Carlsbad, CA, USA), according to the manufacturer’s instructions, and using the same primers.

    other:

    Article Title: Identification of a Privileged Scaffold for Inhibition of Sterol Transport Proteins through the Synthesis and Ring Distortion of Diverse, Pseudo-Natural Products
    Article Snippet: The samples were then heated at the given temperature (Eppendorf Mastercycler ep Gradient S).

    Quantitative RT-PCR:

    Article Title: Effects of cyanobacterium Phormidium nigroviride K3 on zebrafish embryos and genomic insights into its toxic potential.
    Article Snippet: Cyanobacterial blooms in freshwater systems pose significant environmental and public health risks, largely due to their production of toxic secondary metabolites.. This study investigated the effects of Phormidium nigroviride K3 extracts on early zebrafish development, focusing on organismal toxicity and gene expression changes.. Acute toxicity was assessed by monitoring developmental deformities and evaluating relative changes in the expression of six genes associated with xenobiotic metabolism, apoptosis, endoplasmic reticulum stress, and endocrine processes.



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